Organosolvent pretreatment and enzymatic hydrolysis of rice straw for the production of bioethanol |
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Authors: | Raveendran Sindhu Parameswaran Binod Kanakambaran Usha Janu Rajeev K Sukumaran Ashok Pandey |
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Institution: | (1) Centre for Biofuels, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum, 695 019, India; |
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Abstract: | The present study investigates the operational conditions for organosolvent pretreatment and hydrolysis of rice straw. Among
the different organic acids and organic solvents tested, acetone was found to be most effective based on the fermentable sugar
yield. Optimization of process parameters for acetone pretreatment were carried out. The structural changes before and after
pretreatment were investigated by scanning electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR) analysis.
The X-ray diffraction profile showed that the degree of crystallinity was higher for acetone pretreated biomass than that
of the native. FTIR spectrum also exhibited significant difference between the native and pretreated samples. Under optimum
pretreatment conditions 0.458 g of reducing sugar was produced per gram of pretreated biomass with a fermentation efficiency
of 39%. Optimization of process parameters for hydrolysis such as biomass loading, enzyme loading, surfactant concentration
and incubation time was done using Box–Benhken design. The results indicate that acetone pretreated rice straw can be used
as a good feed stock for bioethanol production. |
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