Factors Affecting Ethanol Induced Luminescence in Dark-Treated Chloroplasts |
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Authors: | BJÖRN SIGFRIDSSON |
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Institution: | Department of Plant Physiology, Pack, S-220 07 Lund, Sweden |
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Abstract: | Dark-treated chloroplasts emit light when treated with a high ethanol concentration. The ethanol treatment causes chlorophyll solvation. The light is red and emanates from a singlet excited molecule, probably a chlorophyll peroxide. It is quenched by acetone, sodium dodecyl sulfate treatment of the chloroplasts before the addition of ethanol, boiling, reducing substances and low pH. It is enhanced by ferricyanide and high pH. This is interpreted as a requirement for an organized structure and for an energy transfer system for light emission to occur. |
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