The isolation and physiological analysis of mutants of the moss,Physcomitrella patens,which over-produce gametophores |
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Authors: | N W Ashton D J Cove D R Featherstone |
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Institution: | (1) Botany Department, University of Washington, 98195 Seattle, WA, USA;(2) Present address: Biology Department, Staford University, 94305 Stanford, CA, USA |
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Abstract: | We have compared the effects of cycloheximide (CHI) and two other rapid and effective inhibitors of protein synthesis, pactamycin and 2-(4-methyl-2,6-dinitroanilino)-N-methyl proprionamide (MDMP), on protein synthesis, respiration, auxin-induced growth and H+-excreation of Avena sativa L. coleoptiles. All three compounds inhibit protein synthesis without affecting respiration. The effectiveness of the inhibitors against H+-excretion and growth correlates with their ability to inhibit protein synthesis. Both CHI and MDMP inhibit auxin-induced H+-excretion after a latent period of 5–8 min, and inhibit growth after a 8–10-min lag. These results support the idea that continued protein synthesis is required in the initial stages of the growth-promoting action of auxin.Abbreviations CHI
cycloheximide
- DMSO
dimethyl sulfoxide
- FC
fusicoccin
- IAA
indole-3-acetic acid
- MDMP
2-(4-methyl-2,6-dinitroanilino)-N-methyl proprionamide |
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Keywords: | Auxin Avena Cell elongation Protein synthesis |
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