不结球白菜离体培养与植株再生体系研究

以4个不结球白菜品种为试材,对外植体、苗龄、激素的组配、培养基中琼脂和AgNO3浓度等再生因素进行了筛选优化,并探讨了抗坏血酸(AsA)对不结球白菜不定芽分化的影响。结果显示:以4~7d苗龄的带柄子叶为外植体诱导不定芽效果较好;MN培养基中4mg/L6-BA与0.5mg/LNAA的搭配有利于不定芽形成;琼脂的浓度变化对不定芽分化影响较大,以9g/L琼脂为宜;培养基中添加5~7.5mg/L的AgNO3、0.1~0.5mmol/L的AsA可显著提高不定芽的发生频率和质量。通过不定芽继代培养、生根培养和驯化移栽建立了能够获得较高再生频率的不结球白菜离体再生体系。

In vitro Culture and Regeneration System of Non-heading Chinese Cabbage (Brassica campestris ssp. chinensis Makino)

Effects of factors including explants,seedling age,hormone combinations,agar concentration,AgNO3 and AsA on differentiation of adventitious bud in non-heading Chinese cabbage were evaluated.The results showed that the explants from 4~7 days old seedling are more efficient to induce adventitious buds;the combination of 4 mg/L 6-BA and 0.5 mg/L NAA can improve adventitious buds induction;the culture medium with 9 g/L Agar,5~7.5 mg/L AgNO3 and 0.1~0.5 mmol/L AsA can increase the frequency of differentiation and the quality of buds.A procedure for induction of adventitious buds and regeneration system for non-heading Chinese cabbage was established.Using this system,higher regeneration frequency can be obtained.