多药耐药基因在肝细胞癌中的表达及与p53、PCNA表达的相关性研究

目的　研究肝细胞癌 (HCC)组织中多药耐药基因MDR 1与p5 3蛋白及增殖细胞核抗原 (PCNA)表达的关系 ,旨在从基因水平进一步探讨预测化学治疗效果的可行性。方法　利用免疫组织化学方法 (S P法 )研究 30例肝穿活检的肝癌组织中MDR 1、p5 3和PCNA的表达。结果　 30例肝细胞癌中MDR 1的阳性表达率为 5 6 6 7% ,MDR 1阳性表达与组织学分级无关 (P >0 0 5 )。MDR 1表达与 p5 3、PCNA表达间无相关性 (P >0 0 5 )。结论　通过检测MDR 1基因可以对肝细胞癌病人进行化学治疗敏感性预测 ;肝细胞癌中MDR 1基因表达不依赖于 p5 3和细胞增殖这些因素。

EXPRESSION OF MDR-1 GENE IN HEPATOCELLULAR CARCINOMA AND ITS CORRELATION WITH THE EXPRESSIONS OF p53 AND PCNA

? Objective To correlate the expressionsof p53 and PCNA with the expression of MDR -1gene in hepatocellular carcinoma, and to evalulate the feasibility of predicting the response to chemotherapy with molecular indexs. Method The expressions of MDR-1, p53 and PCNA were examined by means of S-P immunohistochemical technique in 30 cases of hepatocellular carcinoma by puncturing tissues. Result The positive expression rate of MDR -1is 56.67% in the 30 cases of hepatocellular carcinoma, showing no correlation with the histologicalgrade(P>0.05)?There was no correlation between p53, PCNA and MDR -1gene expressions in hepatocellular carcionoma. Conclusion MDR -1provides a useful marker to predict chemotherapy sensitivity the expression of MDR -1gene seems to be independent of cell proliferation or the expression of p53.