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Stability of Collagen During Denaturation |
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| Authors: | Regina Penkova Ivan Goshev Shela Gorinstein and Peter Nedkov |
| Institution: | (1) Department of Chemistry and Biochemistry, Medical University, Pleven, 5800, Bulgaria;(2) Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria;(3) Department of Medicinal Chemistry, School of Pharmacy, Hebrew University-Hadassah Medical School, Jerusalem, 91120, Israel;(4) Bloom Center for Pharmacy, Israel;(5) Department of Pharmaceutical Chemistry, School of Pharmacy, Hebrew University-Hadassah Medical School, Jerusalem, Israel |
| Abstract: | The stability of calf skin collagen (CSC) type I during thermal and chemical denaturation in the presence of glycerol was investigated. Thermal denaturation of type I collagen was performed in the presence of glycerol or in combination with urea and sodium chloride. The denaturation curves obtained in the presence of urea or sodium chloride retained their original shape without glycerol. These curves were shifted upward proportionally to the glycerol concentration in the reaction medium. This means that glycerol and the denaturants act independently. The explanation is based on the difference in the mechanism of their action on the collagen molecule. |
| Keywords: | Collagen glycerol urea sodium chloride stabilization denaturation |
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