Inheritance of plastids in interspecific hybrids of blue spruce and white spruce |
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| Authors: | M. Stine B. B. Sears D. E. Keathley |
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| Affiliation: | (1) Department of Forestry, Michigan State University, 48824 East Lansing, MI, USA;(2) Department of Botany and Plant Pathology, Michigan State University, 48824 East Lansing, MI, USA;(3) Present address: School of Forestry, Wildlife and Fisheries, Louisiana State University, 10803 Baton Rouge, LA, USA |
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| Abstract: | Summary Chloroplast DNA (cpDNA) was purified from blue spruce (Picea pungens Engelm.) and white spruce [P. glauca (Moench) Voss], and was digested with several different restriction endonucleases. Restriction fragment length polymorphisms (RFLPs) were identified that differentiated the cpDNA of both species. Intraspecific conservation of the RFLPs that differentiated each species was confirmed by examining trees from across the natural range of each species. Ten F1 hybrids were examined, and the cpDNA from each showed the banding pattern of the paternal species. Cloned Petunia cpDNA containing part of the rbcL gene hybridized to polymorphic bands, while a cloned maize mtDNA probe of the coxII gene failed to hybridize to any band. |
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| Keywords: | Paternal Inheritance RFLPs Chloroplast DNA Picea Hybrid identification |
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