Affibody-displaying bionanocapsules for specific drug delivery to HER2-expressing cancer cells |
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| Authors: | Takuya Shishido Hiroaki Mieda Sang Youn Hwang Yuya Nishimura Tsutomu Tanaka Chiaki Ogino Hideki Fukuda Akihiko Kondo |
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| Institution: | 1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan;2. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan |
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| Abstract: | A novel HER2-targeted carrier was developed using bionanocapsules (BNCs). Bionanocapsules (BNCs) are 100-nm hollow nanoparticles composed of the l-protein of hepatitis B virus surface antigen. An affibody of HER2 was genetically displayed on the BNC surface (ZHER2-BNC). For the investigation of binding affinity, ZHER2-BNC was incubated with the cancer cell lines SK-BR-3 (HER2 positive), and MDA-MB-231 (HER2 negative). For analysis of HER2 targeting specificity, ZHER2-BNC or ZWT-BNC (without affibody) was incubated with both SK-BR-3 and MDA-MB-231 cells by time lapse and concentration. For the delivery of encapsulated molecules (calcein), fluorescence of ZHER2-BNC mixed with liposomes was also compared with that of ZWT-BNC and nude liposomes by incubation with SK-BR-3 cells. As a result, ZHER2-BNC-liposome complex demonstrated the delivery to HER2-expressing cells (SK-BR-3) with a high degree of specificity. This indicates that genetically engineered BNCs are promising carrier for cancer treatment. |
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