| L-谷氨酰胺高产菌的选育和发酵条件 |
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| 引用本文： | 柴进凯,张伟国.L-谷氨酰胺高产菌的选育和发酵条件[J].生物加工过程,2011,9(1):29-34. |
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| 作者姓名： | 柴进凯 张伟国 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 以嗜乙酰乙酸棒杆菌(Corynebacterium acetoacidophilum)LG-3为出发菌株,经紫外线(UV)和硫酸二乙酯(DES)诱变处理,磺胺胍(SG)、高浓度(NH4)2SO4定向筛选,获得1株谷氨酰胺高产菌株LG-65,在未经优化的条件下摇瓶发酵72 h可产谷氨酰胺43.5 g/L,比出发菌株的产量32.4 g/L提高了34.3%.在此基础上,对其发酵条件进行优化,经72 h摇瓶发酵产量可达47～48 g/L,7 L发酵罐补料分批发酵40 h可达55 g/L.
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| 关 键 词： | 育种 发酵 L-谷氨酰胺 诱变 |
Breeding of L-glutamine high producing strain and its fermentation conditions |
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| CHAI Jinkai,ZHANG Weiguo.Breeding of L-glutamine high producing strain and its fermentation conditions[J].Chinese Journal of Bioprocess Engineering,2011,9(1):29-34. |
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| Authors: | CHAI Jinkai ZHANG Weiguo |
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| Institution: | (Key Laboratory of Industrial Biotechnology of the Ministry of Education,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | The original strain LG-3 was mutated by ultraviolet radiation and diethyl sulfate,and screened in culture dish having high concentration of sulfaguanidine or（NH4）2SO4.A strain LG-65 was obtained for producing 43.5 g/L of L-glutamine.The fermentation conditions were further optimized,the yield reached 47-48 g/L in shaker flask for 72 h and reached 55 g/L cultivated in 7 L fermentor for 40 h. |
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| Keywords: | breeding fermentation L-glutamine mutation |
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