Endophytic fungus <Emphasis Type="Italic">Cladosporium cladosporioides</Emphasis> LF70 from <Emphasis Type="Italic">Huperzia serrata</Emphasis> produces Huperzine A |
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| Authors: | Zhi Bin Zhang Qing Gui Zeng Ri Ming Yan Ya Wang Zheng Rong Zou Du Zhu |
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| Institution: | (1) Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Xuefu Road 576, 336000 Yichun, China;(2) Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Ziyang Road 99, 330022 Nanchang, China; |
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| Abstract: | A strain LF70 endophytic fungus was isolated from the leaves of Huperzia serrata. The fungus was identified as Cladosporium cladosporioides LF70 according to its morphological characteristics and nuclear ribosomal DNA ITS sequence analysis. The strain could produce
Huperzine A (HupA) identified through thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) with
authentic HupA. The amount of HupA produced by this endophytic fungus was quantified to be 56.84 μg/L by HPLC, which was higher
than that of other reported endophytic fungi, Acremonium sp., Blastomyces sp., and Botrytis sp. Acetylcholinesterase inhibition activity of HupA produced by strain LF70 was also similar to authentic HupA in vitro.
Isolation of such a fungus may provide a promising alternative approach to producing HupA, which is used in treating Alzheimer’s
disease and preventing further memory degeneration. |
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