Oxygen evolving membranes and particles from the transformable cyanobacterium Synechocystis sp. PCC6803 |
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| Authors: | R. Burnap H. Koike G. Sotiropoulou L. A. Sherman Y. Inoue |
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| Affiliation: | (1) Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), 351-01 Wako, Saitama, Japan;(2) Department of Biological Sciences, Purdue University, 47907 West Lafayette, IN, U.S.A. |
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| Abstract: | ![]()
Membranes and PS II particles retaining high rates of O2-evolving activity have been isolated from the transformable cyanobacterium, Synechocystis sp. PCC6803. Membranes from cells grown under red light exhibit rates of O2-evolution ranging from 500–700  mole O2/mg chl/h. PS II particles are prepared by a simple procedure involving DEAE column chromatography of detergent extracts obtained by simultaneous treatment of membranes with octylglucoside and dodecylmaltoside. The isolated PS II fraction is enriched in polypeptides immunologically cross-reactive with polypeptides present in core reaction center preparations of spinach, exhibits 77 K fluorescence emission maxima at 685 and 696 nm, but not emission and absorption due to phycobilines and is capable of rates of O2-evolution exceeding 1000 mole O2/mg chl/h.Abbreviations DM dodecyl- -D-maltoside - OG octyl--D-glucoside |
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| Keywords: | non-ionic detergent O2-evolution photosynthesis photosystem II transformable cyanobacterium |
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