HPLC-photodiode array detection analysis of curcuminoids in Curcuma species indigenous to Indonesia |
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| Authors: | Bos Rein Windono Tri Woerdenbag Herman J Boersma Ykelien L Koulman Albert Kayser Oliver |
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| Institution: | Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1,9713 AV Groningen, The Netherlands. R.Bos@rug.nl |
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| Abstract: | An optimized HPLC method with photodiode array detection was developed and applied to analyse the curcuminoids curcumin, demethoxycurcumin, and bis-demethoxycurcumin in rhizomes of Curcuma mangga Val &. v. Zijp, C. heyneana Val. & v. Zijp, C. aeruginosa Roxb. and C. soloensis Val. (Zingiberaceae), indigenous to Indonesia. The method was validated with an isocratic system, a short run time of 10 min and a baseline separation. The curcuminoid content was 0.18-0.47% for C. mangga, 0.98-3.21% for C. heyneana, 0.02-0.03% for C. aeruginosa and 0.40% for C. soloensis. |
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| Keywords: | HPLC‐PAD quantitative determination curcuminoids Curcuma mangga Curcuma heyneana Curcuma aeruginosa Curcuma soloensis Zingiberaceae |
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