| 罗汉松遗传多样性的SCoT分析 |
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| 引用本文： | 韦泳丽,何新华,,罗聪,陈虎.罗汉松遗传多样性的SCoT分析[J].广西植物,2012,32(1):90-93. |
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| 作者姓名： | 韦泳丽 何新华 罗聪 陈虎 |
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| 作者单位： | 1. 广西大学,南宁,530004
2. 广西大学,南宁530004;广西作物遗传改良与生物技术重点实验室,南宁530007 |
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| 基金项目： | 国家科技部农业科技成果转化项目，广西科学研究与技术开发项目 |
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| 摘 要： | 采用SCoT分子标记技术对8份罗汉松种质材料进行遗传多样性研究。结果表明,从80条引物中筛选出10条重复性好、条带清晰的引物进行PCR扩增,共产生136条带,其中多态性带122条(占88.97%),8个罗汉松种质间的遗传相似系数范围在0.39～0.80说明罗汉松的遗传多样性丰富。利用UPGMA进行系统的聚类分析显示,将8份罗汉松材料分为2大类;主成分分析结果与聚类分析结果相一致。可见,利用SCoT分子标记可有效的分析罗汉松种质资源的遗传多样性,为罗汉松种质亲缘关系的鉴别和分类提供理论依据。
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| 关 键 词： | 罗汉松 SCoT 遗传多样性 聚类分析 |
Genetic diversity of podocarpus by SCoT markers |
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| WEI Yong-Li
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HE Xin-Hua
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LUO Cong
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CHEN Hu.Genetic diversity of podocarpus by SCoT markers[J].Guihaia,2012,32(1):90-93. |
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| Authors: | WEI Yong-Li HE Xin-Hua LUO Cong CHEN Hu |
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| Institution: | 1(1.Guangxi University,Nanning 530004,China;2.Guangxi Crop Genetic Improvement and BiotechnologyLaboratory,Guangxi Academy of Agricultural Sciences,Nanning 530007,China) |
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| Abstract: | The genetic diversity of 8 podocarpus germplasms was analyzed by SCoT markers.The results showed that a total of 136 bands were produced by 10 primers,of which 122 bands(88.97%) were polymorphic,and the genetic similarity coefficients were between 0.39-0.80.So there were comparatively great genetic diversity among the podocarpus germplasms.A cluster analysis conducted with UPGMA also showed that the 8 podocarpus germplasms could be classified into 2 groups.The result of principal component analysis(PCA) was the same as the results of UPGMA cluster analysis.SCoT markers can be effectively used to evaluate the genetic diversity of podocarpus germplasms,thus providing a theoretical foundation for the identification and classification of podocarpus germplasms. |
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| Keywords: | docarpus SCoT genetic diversity cluster analysis |
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