Purification and properties of alkaline phosphatase from human polymorphonuclear leukocytes |
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| Authors: | G P Smith T J Peters |
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| Affiliation: | 1. Formulation and Drug Product Development, REGENXBIO Inc., Rockville, MD 20850, USA;2. Process Development, REGENXBIO Inc., Rockville, MD 20850, USA;3. Analytical Development, REGENXBIO Inc., Rockville, MD 20850, USA;4. Manufacturing Sciences, REGENXBIO Inc., Rockville, MD 20850, USA |
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| Abstract: | A procedure for the purification of alkaline phosphatase from human polymorphonuclear leukocytes is described, involving enzyme solubilisation with Triton X-100 and chromatography on DEAE-Sepharose CL 6B and Cibacron Red F = B-Sepharose 4B. The final enzyme preparation was 244-fold purified and was shown to be capable of hydrolysis of a wide range of phosphorylated substates. |
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