| 混合碳源流加对重组毕赤酵母生产碱性果胶酶的影响 |
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| 引用本文： | 汪志浩,张东旭,李江华,堵国成,陈坚.混合碳源流加对重组毕赤酵母生产碱性果胶酶的影响[J].生物工程学报,2009,25(12):1955-1961. |
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| 作者姓名： | 汪志浩 张东旭 李江华 堵国成 陈坚 |
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| 作者单位： | 1. 江南大学工业生物技术教育部重点实验室,无锡,214122
2. 江南大学工业生物技术教育部重点实验室,无锡,214122;江南大学食品科学技术国家重点实验室,无锡,214122 |
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| 基金项目： | 教育部新世纪优秀人才支持计划(No.NCET-07-0378);;国家自然科学基金(No.20836003);;江苏省科技支撑计划(No.SBE200900022);;国家高技术研究发展计划(863计划)(No.2009AA02Z204)资助~~ |
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| 摘 要： | 为提高重组毕赤酵母生产碱性果胶酶(PGL)的产量和生产强度,在诱导期采用多种碳源与甲醇混合添加的模式。实验结果发现:甘油、山梨醇、乳酸与甲醇的混合添加均可以提高PGL的产量,其中山梨醇与甲醇的混合流加效果最为显著。研究表明,通过双碳源混合流加可以提高细胞活力,增强醇氧化酶活力,提高毕赤酵母表达外源蛋白效率。当山梨醇的流速为3.6g/(h·L)时,PGL酶活可达1593U/mL,生产强度为16.7U/(mL·h),比对照分别提高了84.6%和45.2%,实现了碱性果胶酶的高效生产。
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| 关 键 词： | 重组毕赤酵母 碱性果胶酶 混合碳源 流加策略 细胞存活率 醇氧化酶（AOX） |
| 收稿时间： | 2009/9/16 0:00:00 |
Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production |
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| Zhihao Wang,Dongxu Zhang,Jianghua Li,Guocheng Du and Jian Chen.Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production[J].Chinese Journal of Biotechnology,2009,25(12):1955-1961. |
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| Authors: | Zhihao Wang Dongxu Zhang Jianghua Li Guocheng Du and Jian Chen |
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| Institution: | Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | In order to increase the production and productivity of alkaline polygalacturonate lyase (PGL), we studied the mixed carbon sources feeding strategies during the induction phase by recombinant Pichia pastoris GS115. Glycerol, sorbitol or lactic acid co-feeding with methanol all enhanced the PGL production. Among all the feeding strategies, the sorbitol co-feeding strategy was most significant. By using this strategy, the PGL activity and productivity reached 1593 U/mL and 16.7 U/(mL·h). Compared to the control, the enhancements of PGL activity and productivity were 84.6% and 45.2 % respectively, when we set the sorbitol feeding rate at 3.6 g/(h·L). |
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| Keywords: | recombinant Pichia pastoris polygalacturonate lyase mixed carbon sources feeding strategy cell viability alcohol oxidase (AOX) |
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