Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2 |
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| Authors: | Mark D. Burow Zhi-Yuan Chen Tricia M. Mouton James V. Moroney |
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| Affiliation: | (1) Department of Plant Biology, Louisiana State University, 70803 Baton Rouge, LA, USA;(2) Present address: Department of Soil and Crop Sciences, Texas A&M University, 77843 College Station, TX, USA |
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| Abstract: | Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment. |
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| Keywords: | carbonic anhydrase Chlamydomonas CO2 concentrating mechanism gene regulation by CO2 |
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