Immunological enhancement action of endotoxin-free tilapia heat shock protein 70 against Streptococcus iniae |
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Authors: | Ming Chen Rui Wang Liping Li Wanwen Liang Qiuhua Wang Ting Huang Chao Li Jian Li Xi Gan Aiying Lei Weiyi Huang Honglin Luo |
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Institution: | 1. Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China;2. Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China |
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Abstract: | The immunological effects of heat shock proteins (HSPs) had been found in humans and mice, but scarce data of endotoxin-free Hsp70 were reported in tilapia. In the current study, we reported that tHsp70 alone and antigen-tHsp70 compound increased the proliferations of lymphocytes and macrophages, significantly increased the NO release and phagocytotic ability of macrophages (p < 0.05), and enhanced the levels of immune-related genes in lymphocytes and macrophages in a dose- and/or time-dependent manner. On the other hand, tHsp70 not only helped to reduce the proliferation inhibitions induced by the ECP treatment, but also assisted antigens to enhance the vaccine-induced protection against Streptococcus iniae (p < 0.05). We described, for the first time, a critical role of endotoxin-free tHsp70 on activation of tilapia lymphocytes and macrophages post S. iniae exposure and its up-regulation effects on vaccine-induced protection. Our research highlights the immunological enhancement action of Hsp70 in teleost immunity. |
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Keywords: | Tilapia Heat shock protein 70 Streptococcus iniae Lymphocytes Macrophages |
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