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Effect of confounding factors on a phospho-flow assay of ribosomal S6 protein for therapeutic drug monitoring of the mTOR-inhibitor everolimus in heart transplanted patients |
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| Authors: | Maja-Theresa Dieterlen Katja John Susanne Haase Jens Garbade Attila Tarnok Friedrich W Mohr |
| Institution: | 1. HELIOS Clinic, Department of Cardiac Surgery, University Hospital Leipzig, Heart Center, Leipzig, Germany;2. HELIOS Clinic, Department of Pediatric Cardiology, University Hospital Leipzig, Heart Center, Leipzig, Germany |
| Abstract: | Context: Several assays of monitoring immune cell function have been developed to enhance therapeutic drug monitoring. Objective: An in vitro-validated whole-blood assay of phosphorylated ribosomal protein S6 (pS6RP) was evaluated for confounders to monitor the mTOR-inhibitor everolimus (ERL). Materials and methods: Whole blood samples from 87 heart transplant recipients were analyzed for pS6RP-expression in CD3-positive T-cells by phospho-flow analysis. Results: ERL blood concentration, laboratory parameters, co-medications, demographic and clinical data were reviewed. Conclusion: Evaluating the pS6RP-assay revealed that pS6RP is influenced by cyclosporine A (CsA) blood concentration, duration of ERL treatment, co-medication with thiazide diuretics and different metabolic parameters. |
| Keywords: | S6 ribosomal protein everolimus phospho-flow mTOR-inhibitor |