Isolation and properties of a low molecular mass endoglucanase from Trichoderma reesei |
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| Authors: | B Sprey A Uelker |
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| Institution: | Institut für Biotechnologie, Forschungszentrum Jülich GmbH, Jülich, FRG |
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| Abstract: | Abstract Optimal culture conditions for obtaining low molecular mass endoglucanase (EG) from culture fluids of Trichoderma reesei were determined. The purification of this unglycosylated EG in a two-step procedure is described. In contrast to most cellulases, this EG did not bind to ConA-affinity columns. The unglycosylated fraction of the culture fluid proteins was further purified by preparative isoelectric focusing. Conditions relevant to an enzyme assay for this EG were determined (pH optimum 5.8, temperature optimum 52°C). |
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| Keywords: | Trichoderma reesei 1 4-β-Glucan glucanohydrolase Culture conditions |
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