| 氮素形态对番茄根系液泡膜焦磷酸酶活性的影响 |
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| 引用本文： | 朱祝军 Wolfgang PFEIFFER 等.氮素形态对番茄根系液泡膜焦磷酸酶活性的影响[J].Acta Botanica Sinica,2001,43(11):1146-1149. |
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| 作者姓名： | 朱祝军 Wolfgang PFEIFFER |
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| 作者单位： | [1]浙江大学农业与生物技术学院园艺系，杭州310029 [2]奥地利萨尔茨堡大学植物生理研究所，奥地利萨尔茨堡5020 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina ( 39770 440 ) . |
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| 摘 要： | 采用超速梯度离心提取液泡膜微囊，研究硝态氮和铵态氮供应情况下番茄（Lycopersicon esculentum L.)根系液泡膜焦磷酸酶活性的变化。结果表明：供应铵态氮番茄根系专一性液泡膜焦磷酸酶活性比供应硝态氮的高37．7％；供应铵态氮根系液泡膜焦磷酸酶转运质子活力也明显高于供应硝态氮根系。Western blot结果表明，供应铵态氮根系液泡膜焦磷酸酶蛋白表达量也明显增加。
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| 关 键 词： | 番茄 氮素形态 液泡膜 焦磷酸酶 根系 酶活性 |
Effect of Nitrogen Form on the Activity of Tonoplast Pyrophosphatase in Tomato Roots |
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| Authors: | ZHU Zhu_Jun QIAN Ya_Rong Wolfgang PFEIFFER |
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| Institution: | ZHU Zhu_Jun 1,QIAN Ya_Rong 1,Wolfgang PFEIFFER 2 |
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| Abstract: | Effect of nitrate and ammonium on the activity of tonoplast pyrophosphatase (V_PPase) was investigated in the roots of tomato ( Lycopersicon esculentum L.). The results showed that the activity of V_PPase was increased by ammonium nutrition, compared with nitrate nutrition. The H transport of tonoplast vesicles by V_PPase was also stimulated by ammonium nutrition. The result of Western blot indicated that the protein amount of V_PPase was increased by ammonium nutrition. |
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| Keywords: | tomato nitrogen form tonoplast pyrophosphatase |
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