Eye lens proteomics |
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Authors: | W Hoehenwarter J Klose P R Jungblut |
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Institution: | (1) Max Planck Institute for Infection Biology, Core Facility Protein Analysis, Berlin, Germany;(2) Institute for Human Genetics, Charité, Berlin, Germany |
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Abstract: | Summary. The eye lens is a fascinating organ as it is in essence living transparent matter. Lenticular transparency is achieved through
the peculiarities of lens morphology, a semi-apoptotic process where cells elongate and loose their organelles and the precise
molecular arrangement of the bulk of soluble lenticular proteins, the crystallins. The 16 crystallins ubiquitous in mammals
and their modifications have been extensively characterized by 2-DE, liquid chromatography, mass spectrometry and other protein
analysis techniques. The various solubility dependant fractions as well as subproteomes of lenticular morphological sections
have also been explored in detail. Extensive post translational modification of the crystallins is encountered throughout
the lens as a result of ageing and disease resulting in a vast number of protein species. Proteomics methodology is therefore
ideal to further comprehensive understanding of this organ and the factors involved in cataractogenesis. |
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Keywords: | : Eye lens – Proteomics – Crystallins – 2-DE – Liquid chromatography – Mass spectrometry |
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