Immunoproteomically identified GBAA_0345, alkyl hydroperoxide reductase subunit C is a potential target for multivalent anthrax vaccine |
| |
Authors: | Yeon Hee Kim Kyung Ae Kim Yu‐Ri Kim Min Kyung Choi Hye Kyeong Kim Ki Ju Choi Jeong‐Hoon Chun Kiweon Cha Kee‐Jong Hong Na Gyong Lee Cheon‐Kwon Yoo Hee‐Bok Oh Tae Sung Kim Gi‐eun Rhie |
| |
Institution: | 1. Division of High‐risk Pathogen Research, Korea National Institute of Health, Chungbuk, Republic of Korea;2. School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea;3. Department of Bioscience & Biotechnology, Institute of Bioscience, Sejong University, Seoul, Republic of Korea;4. Division of Respiratory Viruses, Korea National Institute of Health, Chungbuk, Republic of Korea |
| |
Abstract: | Anthrax is caused by the spore‐forming bacterium Bacillus anthracis, which has been used as a weapon for bioterrorism. Although current vaccines are effective, they involve prolonged dose regimens and often cause adverse reactions. High rates of mortality associated with anthrax have made the development of an improved vaccine a top priority. To identify novel vaccine candidates, we applied an immunoproteomics approach. Using sera from convalescent guinea pigs or from human patients with anthrax, we identified 34 immunogenic proteins from the virulent B. anthracis H9401. To evaluate vaccine candidates, six were expressed as recombinant proteins and tested in vivo. Two proteins, rGBAA_0345 (alkyl hydroperoxide reductase subunit C) and rGBAA_3990 (malonyl CoA‐acyl carrier protein transacylase), have afforded guinea pigs partial protection from a subsequent virulent‐spore challenge. Moreover, combined vaccination with rGBAA_0345 and rPA (protective antigen) exhibited an enhanced ability to protect against anthrax mortality. Finally, we demonstrated that GBAA_0345 localizes to anthrax spores and bacilli. Our results indicate that rGBAA_0345 may be a potential component of a multivalent anthrax vaccine, as it enhances the efficacy of rPA vaccination. This is the first time that sera from patients with anthrax have been used to interrogate the proteome of virulent B. anthracis vegetative cells. |
| |
Keywords: | Bacillus anthracis Immunoproteomics Alkyl hydroperoxide reductase subunit C Microbiology Vaccine |
|
|