马齿苋多糖对肠道微生态失调小鼠的调整作用研究

目的研究应用马齿苋多糖对肠道微生态失调小鼠进行调整治疗,达到从微生态学角度防治感染的目的。方法应用林可霉素灌胃建立肠道微生态失调小鼠模型,然后用马齿苋多糖进行治疗,同时设正常对照组、阳性对照组和阴性对照组,于给药7 d后处死小鼠,进行肠道菌群定量、肠内容物挥发性脂肪酸检测及肠黏膜电镜观察。结果林可霉素灌胃3 d后,小鼠肠道菌群失调,肠内容物挥发性脂肪酸含量明显下降,肠黏膜损伤严重。持续7 d治疗后,治疗组小鼠肠道双歧杆菌和乳酸杆菌数量明显上升,肠内容物挥发性脂肪酸含量明显上升,损伤的肠黏膜基本修复。结论应用林可霉素可以成功建立肠道微生态失调动物模型;马齿苋多糖具有扶植肠道正常菌群生长,调整菌群失调,防治感染的作用,是理想的中药微生态调节剂。

Effects of Portulaca polysaccharide on the dysbacteria in intestinal tract of mice

Objective To explore the regulation of Portulaca polysaccharide in intestinal microbe dysbiosis mice. Method The intestinal microbe dysbiosis mice were established by intragastric administration of lincomycin, and then treated with the decoction of Portulaca polysaccharide. At the same time, normal control, positive control and spontaneous recovery group were set up. The mice were sacrificed after being treated 7 days and the intestinal microbial population, the volatile fatty acid in the blindguts of mice were detected, and bowel tissue electronmicroscopic changes were performed. Re- sult After intragastrie administration for 3 days, the intestinal microbial flora of the mice was disturbanced, the volatile fatty acid in the blindguts of mice were decreased, and intestinal mucosa were injured seriously. After treatment for 7 days, the number of Bifidobacterium and Bacteroid in Portulaca polysaccharide treated group were higher than that of spontaneous recovery group, the volatile fatty acid in the blindguts of mice were increased, and the injured intestinal mucous membrane was almost recovered. Conclusion The animal model of microccology dysbiosis can be induced by oral Lincomycin; Portulaca polysaccharide is an ideal microccological modulator.