| 用核酸斑点杂交法比较油桐尺蠖核型多角体病毒与某些昆虫杆状病毒的同源性 |
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| 引用本文： | 孟小林,刘年翠,丁清泉.用核酸斑点杂交法比较油桐尺蠖核型多角体病毒与某些昆虫杆状病毒的同源性[J].病毒学报,1988(4). |
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| 作者姓名： | 孟小林 刘年翠 丁清泉 |
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| 作者单位： | 武汉大学病毒系
(孟小林，刘年翠)，中国科学院武汉病毒研究所(丁清泉) |
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| 摘 要： | 有关核型多角体病毒基因同源性的研究报道不多,结果也不甚一致。目前,国内外普遍用SDS-PAGE法分析昆虫杆状病毒结构多肽,以限制性内切酶谱法测定基因组大小,借此区分来自不同宿主的病毒株,和基因组密切相关
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| 关 键 词： | 斑点杂交 昆虫杆状病毒 油桐尺蠖核型多角体病毒 同源性比较 |
HOMOLOGY OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS DNA WITH DNA OF OTHER INSECT BACULOVIRUSES BY DOT-HYBRIDIZATION |
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| Meng Xiaolin Liu Niancui Ding Qingquan.HOMOLOGY OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS DNA WITH DNA OF OTHER INSECT BACULOVIRUSES BY DOT-HYBRIDIZATION[J].Chinese Journal of Virology,1988(4). |
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| Authors: | Meng Xiaolin Liu Niancui Ding Qingquan |
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| Institution: | Meng Xiaolin Liu Niancui Ding Qingquan Department of Virology,Wuhan University,Wuhan Wuhan Institute of Virology,Academia Sinica,Wuhan |
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| Abstract: | BsNPV-DNA probe was prepared by nick translation. Dot hybridization was carried out on the nitricellulose membrane with six other insect bacu-loviruses, Buzura thibetaria NPV (BtNPV), Ectropis obliqua hypulina NPV (EoNPV), Chihuo zao Yang NPV(CzNPV), Philosamia cynthia ritina NPV (PcNPV) Eupterote chinensis Leech NPV (EcNPV ) and Pieris rapae GV ( PrGV ) . The results obtained by dot hybridization indicated that BtNPV, EoNPv and CzNPV showed positive reaction and pcNPV, EcNPV and PrGV showed negative reaction with BsNPV-DNA probe. |
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| Keywords: | Dot hybridization Insect baculoviruses Buzura suppressaria Nuclear Polyhedrosis Virus Homology |
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