IL-17 is involved in bone resorption in mouse periapical lesions |
| |
Authors: | Fumishige Oseko Toshiro Yamamoto Yuki Akamatsu Narisato Kanamura Yoichiro Iwakura Jiro Imanishi Masakazu Kita |
| |
Institution: | Departments of Microbiology;and Dental Medicine, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto 602-8566;and Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan |
| |
Abstract: | Periapical lesions are induced by bacterial infection of the dental pulp and result in destruction of the surrounding alveolar bone. Although various immunological studies concerning periapical bone resorption have been reported, the role of cytokines in the formation of periapical lesions remains unclear. In this study, the role of IL-17A in periapical lesions in mice was investigated. Normal C57BL/6, IFN-γ?/?, TNF-α?/?, and IL-17A?/? mice were subjected to pulp exposure and infected with Prevotella intermedia (ATCC25611) and Porphyromonas gingivalis (ATCC33277) in the mandibular first molar. Periapical lesions were determined by μCT on day 21 after infection, and 3D visual construction was performed using 3D picture quantification software. The expression of IL-17A mRNA in periapical lesions was determined by the RT-PCR and real-time RT-PCR method. Periapical lesions developed in wild-type, IFN-γ?/?, and TNF-α?/? mice after infection with P. intermedia and P. gingivalis . However, periapical lesions were not observed in IL-17A?/? mice. The expression of IL-17A mRNA was significantly induced in periapical lesions of wild-type mice after infection. These results suggest that IL-17A, but not IFN-γ or TNF-α, plays an important role in the formation of periapical lesions. |
| |
Keywords: | interferon interleukin 17 micro-computed tomography periapical lesion |
|
|