|
|||||
|
|
Isolation and Fluorescence Spectrum of Cardiac Myosin from Pig Heart |
|
| Authors: | Yang Liu Buhai Li Xiaomei Sun Aihua Lin |
| Affiliation: | 1. School of Life Science and Technology, Wuhan University of Science and Technology Zhongnan Branch, 430223, Wuhan, Hubei, People’s Republic of China 2. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South-Central University for Nationalities, 430074, Wuhan, Hubei, People’s Republic of China 3. Key Laboratory of Biological Technology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, 430074, Wuhan, Hubei, People’s Republic of China |
| Abstract: | Cardiac myosin is the most abundant and bioactive protein presented in heart tissue. In this work, a simple method was described for preparation of high purity cardiac myosin for further research. With the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 U/mg. The purification factor was 4.9-fold with an activity recovery of 42.1%. In addition, the investigation of fluorescence spectrum shown that the emission intensity of cardiac myosin has a dramatic decrease under the influence of an acidic environment or at temperatures higher than 30 °C, which could influence the normal physiological function of cardiac myosin. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |