环孢菌素8562的研究

中国弯颈霉Tolypocladium sinense C.L.Li 8562培养物用乙酸乙酯萃取,减压浓缩,获粗提品,经硅胶柱层析分离,葡聚糖凝胶LH-20纯化,活性炭脱色,得到2个成份,8562-成份1,8562-成分2,以成分1为主。经紫外光谱、红外光谱、高效液相色谱、质谱、核磁共振氢谱等分析表明8562-成份1、8562-成份2与环孢菌素A、环孢菌素C同质。8562-成分1的抗真菌试验显示了对半知菌和酵母菌具有明显拮抗活性,最低抑菌浓度(MIC)为2—150μg/ml。

STUDIES ON CYCLOSPORIN 8562

Cyclosporin 8562 was produced by Tolypocladium sinense C. L. Li strain 8562 which was isolated from fresh material Cordyceps sinensis (Berk.) Sacc, collected from Diqing Tibetan Autonomopus Prefecture, Yunnan province. The cultures of Tolypocladium sinense C. L. Li strain 8562 was extracted with an equal volume of acetic ether and concentrated to dry under reduced pressure. The light yellow crude samples was separated by silica gel column chromatography using acetic ether water saturation as the eluent. The crude compound was separated into two components: 8562-component 1 and 8562-component 2. Each component was then further purified by sephadex LH-20 chromatography using methanol as the eluent. They are as colourless amorphous power. Purified sample 8562-component 1 and 8662-co-mponent 2 were identified as Cyclosporin A and cyclosporin C respectively by TLC, UV, IR, HPLC, FABMS and 1H-NMR. It could be concluded that the 8562-component 1 and 8562-component 2 of the compound produced by Tolypocladium sinense was identical with cyclosporin A and cyclosporin C. The antiobiosis of 8562-component 1 was investgated and compared with that of known cyclosporin A. The 8562-component 1 was shown in this test to have a obvious antifungal activity against some imperfect fungi and yeasts.