The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae |
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| Authors: | Abdel-Mottaleb Yousra Clynen Elke Jalali Amir Bosmans Frank Vatanpour Hossein Schoofs Liliane Tytgat Jan |
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| Affiliation: | Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, P.O. Box 922, 3000 Leuven, Belgium. |
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| Abstract: | The very first member of K(+) channels toxins from the venom of the Iranian scorpion Odonthobuthus doriae (OdK1) was purified, sequenced and characterized physiologically. OdK1 has 29 amino acids, six conserved cysteines and a pI value of 4.95. Based on multiple sequence alignments, OdK1 was classified as alpha-KTx 8.5. The pharmacological effects of OdK1 were studied on six different cloned K(+) channels (vertebrate Kv1.1-Kv1.5 and Shaker IR) expressed in Xenopus laevis oocytes. Interestingly, OdK1 selectively inhibited the currents through Kv1.2 channels with an IC50 value of 183+/-3 nM but did not affect any of the other channels. |
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| Keywords: | ESI-Q-TOF MSMS, electrospray ionization hybrid quadrupole time of flight tandem mass spectrometry HPLC, high performance liquid chromatography KTx, K+ channel toxin Kv, voltage-gated K+ channel TFA, trifluoro acetic acid 4-AP, 4-aminopyridine |
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