Salt Stress-Induced Cytoplasmic Acidification and Vacuolar Alkalization in Nitellopsis obtusa Cells : In VivoP-Nuclear Magnetic Resonance Study |
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Authors: | Katsuhara M Kuchitsu K Takeshige K Tazawa M |
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Institution: | Department of Biology, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan. |
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Abstract: | Time courses of cytoplasmic and vacuolar pH changes under salt stress were monitored by in vivo31P-nuclear magnetic resonance spectroscopy in intact cells of Nitellopsis obtusa. When cells were treated with 100 millimolar NaCl for 2 hours, the cytoplasmic pH deceased from 7.2 to 7.0, while the vacuolar pH increased from 4.9 to 5.2. This salt-induced breakdown of the pH gradient between the cytoplasm and the vacuole was also confirmed through direct measurements of change in vacuolar pH with a micro-pH electrode. We speculate that the intracellular pH changes induced by the salt stress mainly results from the inhibition of the H+-translocating pyrophosphatase in the vacuolar membrane, since this H+-translocating system is sensitive to salt-induced increase in the cytoplasmic Na+] and a simultaneous decrease in the cytoplasmic K+]. Since disturbance of the cytoplasmic pH value should have serious consequences on the homeostasis of living cells, we propose that the salt-induced intracellular pH changes are one of initial and important steps that lead to cell death. |
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