The role of ethylene and reducing agents on anther culture response of tetraploid potato (Solanum tuberosum L.) |
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Authors: | Taina Tiainen |
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Institution: | (1) Agricultural Research Centre, Institute of Plant Breeding, SF-31600 Jokioinen, Finland;(2) Present address: Pakilantie 5 A 14, SF-00630 Helsinki, Finland |
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Abstract: | Summary The role of ethylene in embryogenesis of cultured potato anthers was studied indirectly by testing various substances known to affect ethylene formation. The reducing agents ascorbic acid and L-cysteine prevented browning of anther cultures and significantly stimulated embryogenesis. Embryogenesis was also promoted by the use of the ethylene inhibitors AgNO3 and n-propyl-gallate and by the polyamines spermidine and putrescine. The use of the ethylene releasing compound ethrel significantly inhibited embryogenesis.Abbreviations MS
Murashige & Skoog
- PVP
polyvinylpyrrolidone
- MW
molecular weight
- ACC
1-aminocyclopropane-1-carboxylic acid
- ethrel
2-chloroethylphosphonic acid (ethephon) |
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Keywords: | Anther culture Solanum tuberosum reducing agents ethylene inhibitors polyamines polyamines |
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