Microencapsulated Chromatophores for the Production of ATP |
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Authors: | Koji Sode Atsushi Saito Masayasu Suzuki Kazuhito Kajiwara Isao Karube |
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Affiliation: | a Research Center for Advanced Science and Technology, University of Tokyo, Muguro-ku, Tokyo, Japanb Research Laboratory of Resources Utilization., Tokyo Institute of Technology, Midori-ku, Yokohama, Japan |
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Abstract: | Chromatophores, organelles for photophosphorylation in non-sulfur purple photosynthetic bacteria, were microencapsulated and utilized in ATP production. The microcapsules were formed by photocrosslinking with trimethylolpropane triacrylate (TMPTA). In batch experiments chromatophores microencapsulated in TMPTA capsules were repeatedly used in ATP production for more than 5 times. Continuous ATP production was then undertaken. ATP was produced at a production rate of 14 μmol h-1 L-1 over 200 hrs. The yield (from ADP to ATP) was 35%. The total amount of ATP produced was 0.7 mM (μM Bchl)-1. Therefore, this microencapsulation method was found to be suitable for the continuous ATP production using chromatophores. |
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Keywords: | ATP production Chromatophores Microencapsulation Photophosphorylation Immobilization |
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