甲醇溶液对木瓜蛋白酶催化活性的影响

试验结果表明,木瓜蛋白酶在一定浓度甲醇溶液中水解酪蛋白的活性显著上升;动力学测定表明,该酶在甲醇溶液中Km下降;在甲醇溶液中木瓜蛋白酶催化酪蛋白水解反应的最适pH为8.5,最适温度为70℃;紫外差示光谱研究表明,在甲醇溶液作用下木瓜蛋白酶的二级结构发生了变化;荧光发射光谱研究表明,木瓜蛋白酶在甲醇溶液中发射峰位向低波长移动,荧光峰值明显增高。

Effect of Methanol on Catalytic Activity of Papain

The catalytic activity of papain could increase rapidly when treated with methanol. Results of kinetics experiments indicated that the value of Km and Vmax declined. The optimum pH of methanol solution was 8.5 and the optimum temperature was 70℃ for catalytic activity of papain. The experimental results of differential ultraviolet spectra of papain showed that the conformation of papain changed in methanol solution. The fluorescence emission spectra showed that the fluorescence intensity of papain increased and the fluorescence emission peak changed to lower wavelength in methanol solution.