Osmotic hemolysis contrasted with freeze-thaw hemolysis |
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Authors: | D B Pribor |
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Affiliation: | Biology Department, The University of Toledo and Research Associate, Institute of Medical Research of The Toledo Hospital, Toledo, Ohio 43606 USA |
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Abstract: | Red blood cell ghosts resulting from osmotic hemolysis in the presence of Mg ions (Mg ghosts) and ghosts resulting from slow freeze-thaw process (freeze-thaw ghosts) differ in many respects: (1) Mg ghosts spontaneously take on the disc shape immediately after hemolysis and resuspension in buffered salt solution; whereas freeze-thaw ghosts are spherical; (2) Mg ghosts appear to be less hemolyzed than freeze-thaw ghosts; (3) washed and cold-stored Mg ghosts contract or become biconcave discs when exposed to 30 μmoles of ATP/109 ghosts at 37 °C; whereas freeze-thaw ghosts under similar conditions break up into microspheres and membrane filaments; (4) Mg ghosts become crenated discs and spheres when rehemolyzed and resuspended in buffered salt solution; whereas freeze-thaw ghosts tend to fragment; (5) the ATPase activity of Mg ghosts, particularly the nontransport ATPase activity, is considerably less than that of freeze-thaw ghosts. |
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