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Ribosomal protein genes of yeast contain intervening sequences
Authors:Godfried H.P.M. Bollen  Constance M.T. Molenaar  Louis H. Cohen  Mary M.C. van Raamsdonk-Duin  Willem H. Mager  Rudi J. Planta
Affiliation:Biochemisch Laboratorium, Vrije Universiteit, 1081 HV AmsterdamThe Netherlands
Abstract:From a colony bank of HindIII-generated yeast DNA fragments we have isolated a number of recombinant DNAs carrying genes for ribosomal proteins (e.g., S10, S16A, S20, S24, S31, S33, L16, L25 and L34) of the yeast Saccharomyces carlsbergensis. By electron microscopic analysis of the R-loops formed between various DNA fragments and yeast mRNA, we could locate the ribosomal protein genes on the physical maps of the cloned DNA fragments. The R-loop structures observed indicate that a number of the ribosomal protein genes contain an intervening sequence.
Keywords:Recombinant DNA  Saccharomyces carlsbergensis  restriction maps  R-loop electron microscopy  ribosomal protein genes  kb  kilobase pairs  Pipes  piperazine-Jv', N'-bis-2-ethanesulfonic acid  SDS  sodium dodecyl sulfate
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