Differential colorimetric analysis of some deoxyribosyl compounds |
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| Affiliation: | University of Oviedo, Oviedo, Spain;University of Newcastle, Callaghan, NSW, Australia;CSIRO, Energy Division, Newcastle, NSW, Australia;University of Newcastle, Callaghan, NSW, Australia;Drève de Nivelles, Brussels, Belgium;University of Rome La Sapienza, Rome, Italy;Burgess Analytical Consultancy Limited, Barnard Castle, Co Durham, United Kingdom;Medical University of Lublin, Lublin, Poland |
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| Abstract: | Simple procedures are described for the estimation of purine deoxyribonucleosides and deoxyribonucleotides. The nucleosides are hydrolytically cleaved at pH 2 and oxidized by periodate at room temperature. Under such conditions, the nucleotides are not oxidized, but they may be hydrolyzed and oxidized by periodate at 70 °. In each instance, the resulting malonaldehyde is estimated colorimetrically with thiobarbituric acid. Aqueous solutions containing free deoxyribose, free deoxyribose 5-phosphate, purine deoxyribonucleosides, and purine deoxyribonucleotides may be assayed for each deoxyribosyl species. |
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