A gas-counting method for determination of carbon-14 of proteins labeled with both carbon-14 and iodine-131 |
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| Affiliation: | 1. Editor chefe, Jornal de Pediatria, Brasil;2. Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brasil;1. New York Medical College, Valhalla, Estados Unidos;2. Maria Fareri Children''s Hospital, Valhalla, Estados Unidos;1. Instituto da Criança, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brasil;2. Unidade de Nefrologia Pediátrica, Instituto da Criança, Faculdade de Medicina, Universidade de São Paulo (USP), São Paulo, SP, Brasil;1. The Children''s Bladder Clinic, Reino Unido;2. Evelina London Children''s Hospital, Londres, Reino Unido;3. Guy''s and St Thomas’ NHS Foundation Trust, Londres, Reino Unido;4. International Children''s Continence Society, Chappaqua, Estados Unidos;1. College of Life Sciences, Shenyang Normal University, Shenyang 110034, Liaoning, China;2. Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;1. College of Life Sciences, Shenyang Normal University, Shenyang, 110034, China;2. Key Laboratory of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China;3. Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China |
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| Abstract: | The Bernstein-Ballentine gas-counting method has been used for determining the activity of C14-labeled carbon dioxide produced from protein labeled with both C14 and I131. A slight modification of the oxidation mixture used by Van Slyke et al. (5) makes the Van Slyke combustion procedure (1, 4) applicable to the production of carbon dioxide from doubly labeled protein. The ninhydrin technique (2) is also suitable for releasing C14-labeled carbon dioxide from hydrolyzates of protein labeled with both C14 and I131. |
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