An improved microelectrophoresis apparatus and technique for studying biological cell surfaces

A modified form of microelectrophoresis apparatus and technique for studying biological cell surfaces is described. The design of apparatus has been simplified thereby increasing the ease of assembly and manipulation. This is the first description of a microelectrophoresis cell using a rectangular observation chamber, conforming to the theoretical requirements in which the temperature can be accurately controlled. It thus makes an advance in design for it permits experimental verification of the theoretical mobility temperature relation.The apparatus has been extensively tested by statistically designed experiments. These revealed the main sources of errors, which have been erradicated by modifications and the establishment of a standard experimental procedure. Using this procedure, results obtained day to day and over a period of weeks are reproducible to ±3%.