Toxicity of a heterologous leucyl-tRNA (anticodon CAG) in the pathogen Candida albicans: in vivo evidence for non-standard decoding of CUG codons |
| |
Authors: | Christoph E Leuker and Joachim F Ernst |
| |
Institution: | (1) Institut für Mikrobiologie, Universitätsstr. 1/Geb. 26.12, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany |
| |
Abstract: | Plasmids containing derivatives of the Saccharomyces cerevisiae leucyl-tRNA (tRNA3
3
Leu
) gene that vary in anticodon sequence were constructed and transformed into the pathogen Candida albicans and S. cerevisiae. C. albicans could readily be transformed with plasmids encoding leucyl-tRNA genes with the anticodons CAA and UAA (recognizing the codons UUG and UUA) and expression of the heterologous tRNALeu could be demonstrated by Northern RNA blotting. In contrast, no transformants were obtained if the anticodons were UAG (codons recognized CUN, UUR) and CAG (codon CUG), indicating that the insertion of leucine at CUG codons is toxic for C. albicans. All tRNALeu-encoding plasmids transformed S. cerevisiae with equally high efficiencies. These results provide in vivo evidence that non-standard decoding of CUG codons is essential for the viability of C. albicans. |
| |
Keywords: | Candida albicans Codon usage Leucyl-tRNA Heterologous gene expression |
本文献已被 SpringerLink 等数据库收录! |