Sinapoylglucose: malate sinapoyltransferase activity in seeds and seedlings of rape |
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Authors: | Dieter Strack Brian E Ellis Walter Gräwe Jürgen Heilemann |
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Institution: | (1) Institut für Pharmazeutische Biologie der Technischen Universität Braunschweig, Mendelssohnstrasse 1, D-3300 Braunschweig, Germany;(2) Department of Plant Science, University of British Columbia, 2357 Main Mall, V6T 2A2, Vancouver, B.C., Canada;(3) Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, 41 Köln, Germany |
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Abstract: | Protein preparations from seeds and seedlings (cotyledons) of rape (Brassica napus subsp. napus L.] DC.) catalyzed the transfer of sinapic acid from 1-Osinapoyl--glucose to malate in the formation of O-s-inapoylmalate. The enzyme involved, 1-O-sinapoyl--glucose: l-malate O-sinapoyltransferase (SMT; EC 2.3.1), catalyzes the key step in the overall conversion of the seed constituent sinapine (O-sinapoylcholine) to the accumulating O-sinapoylmalate by way of the intermediate 1-O-sinapoyl--glucose. The present paper describes this phenomenon focussing on SMT activity.Abbreviations Sin-Glc
1-O-sinapoyl--glucose
- Sin-Mal
O-sinapoylmalate
- SMT
1-O-sinapoyl--glucose: l-malate sinapoyltransferase (EC 2.3.1)
This work was supported by the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie and the Ontario Ministry of Agriculture and Food. |
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Keywords: | Acyltransferase Brassica (cotyledon seed) Sinapic acid ester Sinapine Sinapoylmalate Sina poyltransferase |
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