|
|||||
|
|
An improved HPLC assay of the substrate and products of the isopenicillin N synthase |
|
| Authors: | Boudewijn D Meesschaert Jesús Cortés Emilio Alvarez Paloma Liras Juan F Martín |
| Institution: | (1) Departamento de Microbiología, Facultad de Biología, Universidad de León, Leon, Spain;(2) Present address: K.I.H.W.-V1., Zeedijk 101, 8400 Oostende, Belgium |
| Abstract: | Summary An improved HPLC method is described to separate isopenicillin N from its penicilloic acid and from the reduced and oxidized forms of  -(L- -amino-adipyl)-L-cysteinyl-D-valine and of DTT. When applied to assay the activity of isopenicillin N synthase only isopenicillin N and its penicilloic acid could be detected. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |