Morphological,pathological and molecular characterisation of rice sheath blight disease causal organism Rhizoctonia solani AG-1 IA in Egypt |
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| Authors: | Rabie A S El-Shafey Mona M Saleh Abdelaziz M Tahoon Amero A Emeran |
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| Institution: | 1. Rice Research and Training Center, Field Crops Research Institute, Agricultural Research Center, Kafrelshiekh, Egypt;2. relshafey13@yahoo.com;4. Rice Pathology Department, Plant Pathology Research Institute, Agricultural Research Center, Kafrelsheikh, Egypt;5. Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt |
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| Abstract: | Rice sheath blight disease caused by Rhizoctonia solani is considered a distractive soil-borne disease of rice production worldwide. The study aimed to determine the causal organism of sheath blight symptoms in Egyptian rice fields. Sheath blight symptoms were first observed in a small area during 2013, 2014 and 2016 seasons, later in a wide area of rice fields in 2016 to 2018 seasons. Pathogen identification was carried out based on morphological traits and internal transcribed spacer sequencing. Thirty-six isolates were identified as R. solani fungus. The isolates exhibited a wide range of variability in their morphological traits and virulence patterns. Five isolates were sequenced and aligned with Chinese isolates with 75–100% identity. This is the first report of R. solani AG-1 IA that associated with rice sheath blight in Egypt. Initiate a breeding program for disease resistance and integrated disease management procedures are important to keep the disease under control. |
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| Keywords: | Rice sheath blight Rhizoctonia solani AG-1 IA identification ITS region Egypt |
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