Molecular genetics of rotifers: preliminary restriction napping of the mitochondrial genome of Brachionus plicatilis |
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Authors: | King Charles E |
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Institution: | (1) Dept. of Zoology, Oregon State University, 97331 Corvallis, OR, U.S.A. |
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Abstract: | Methods are presented to extract and purify mitochondrial DNA from the rotifer Brachionus plicatilis. The mtDNA obtained is of sufficient purity for digestion with restriction endonucleases. EcoR I restriction patterns are presented for 4 geographically separated clones. A restriction map based on digestion with 5 different restriction enzymes is included for one of these clones. Finally, use of mtDNA analysis for studies on the population structure and biogeography of rotifers is discussed. |
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Keywords: | mitochondrial DNA genetic variation restriction mapping rotifers Brachionus plicatilis |
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