| 植物盐胁迫应答蛋白质组学研究的技术策略 |
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| 作者姓名： | Zhang H Dai SJ |
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| 作者单位： | 东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040;东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨150040 |
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| 基金项目： | 国家自然科学基金项目(31071194); 黑龙江省杰出青年科学基金项目(JC201011); 中央高校基本科研业务费专项(DL09DA03); 国家博士后科学基金面上项目(第48批)资助 |
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| 摘 要： | 土壤盐渍化是限制植物生长和分布的关键因素之一.揭示植物响应盐胁迫的分子机理是借助分子生物学手段提高植物耐盐性的基础,也是当前植物生理与分子生态学研究的热点问题.高通量的蛋白质组学技术体系包括双向电泳技术、蓝色自然胶电泳技术、双向荧光差异凝胶电泳技术、液相色谱技术,以及各种生物质谱技术,已经被广泛应用于植物应答盐胁迫研究,为解析植物耐盐分子机制提供了重要信息.本文综述了应用于植物盐胁迫响应蛋白质组学研究的技术策略.
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| 关 键 词： | 植物 蛋白质组学 盐胁迫 技术策略 |
Technical strategies in the research of plant salt-responsive proteomics: a review |
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| Zhang H,Dai SJ.Technical strategies in the research of plant salt-responsive proteomics: a review[J].Chinese Journal of Applied Ecology,2011,22(8):2201-2210. |
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| Authors: | Zhang Heng Dai Shao-jun |
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| Institution: | ZHANG Heng1,2,DAI Shao-jun1,2(1Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin 150040,China,2Ministry of Education Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field,China). |
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| Abstract: | Soil salinization is one of the key factors limiting plant growth and distribution.To explore plant molecular salt-responsive mechanisms is the basis of enhancing plant salt tolerance in virtue of molecular biological techniques,and also,the hotspot of plant physiology and molecular ecology.High throughput proteomics approaches include two-dimensional electrophoresis(2-DE),blue-native/SDS-PAGE(BN/SDS-PAGE),two-dimensional fluorescence difference gel electrophoresis(2D DIGE),liquid chromatography(LC),and var... |
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| Keywords: | plant proteomics salt stress technical strategy |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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