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| 三株降解芘的戈登氏菌鉴定及其降解能力 | |
| 作者姓名： | Hu FC Li XY Su ZC Wang XJ Zhang HW Sun JD |
| 作者单位： | 1. 沈阳农业大学土地与环境学院,沈阳,110161;森林与土壤生态国家重点实验室,中国科学院沈阳应用生态研究所,沈阳,110164 2. 森林与土壤生态国家重点实验室,中国科学院沈阳应用生态研究所,沈阳,110164 3. 沈阳农业大学土地与环境学院,沈阳,110161 |
| 基金项目： | 中国科学院知识创新工程重要方向项目(KSCX2-YW-G-053-2)资助 |
| 摘 要： | 从沈抚灌区多环芳烃污染土壤中筛选出的芘降解菌D44、D82S和D82Q,经形态观察、生理生化试验和16S rDNA序列分析确定均为戈登氏菌属(Gordonia sp.).3株菌的最适生长pH值均为7,当pH值低于5或高于9时,生长均受到明显抑制.降解试验表明,3株菌能以芘、苯并芘、蒽、萘、菲和荧蒽为唯一碳源和能源生长.经过7 d的培养,3株菌对初始浓度为100 mg.L-1的芘的降解率均在65%以上,对初始浓度为50 mg.L-1的苯并芘的降解率分别为79.6%、91.3%和62.8%.通过PCR检测发现D82Q和D82S含有烷烃单加氧酶基因alkB. |
| 关 键 词： | 戈登氏菌 多环芳烃 芘 生物降解 |
Identification and degradation capability of three pyrene-degrading Gordonia sp. strains |
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| Hu FC,Li XY,Su ZC,Wang XJ,Zhang HW,Sun JD.Identification and degradation capability of three pyrene-degrading Gordonia sp. strains[J].Chinese Journal of Applied Ecology,2011,22(7):1857-1862. | |
| Authors: | Hu Feng-chai Li Xin-yu Su Zhen-cheng Wang Xiu-juan Zhang Hui-wen Sun Jun-de |
| Institution: | HU Feng-chai1,2,LI Xin-yu2,SU Zhen-cheng2,WANG Xiu-juan2,ZHANG Hui-wen2,SUN Jun-de1(1College of Land and Environment,Shenyang Agricultural University,Shenyang 110161,China,2State Key Laboratory of Forest and Soil Ecology,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110164,China) |
| Abstract: | Three pyrene-degrading bacterial strains named D44,D82S and D82Q were isolated from PAHs-contaminated soil in Shenfu Irrigation Area of Shenyang,Northeast China.The strains were identified as Gordonia sp.,based on the morphological observation,physiological and biochemical identification,and phylogenetical analysis of 16S rDNA sequences.For all the three stains,their optimal pH was 7,and their growth was obviously inhibited when the pH was lower than 5 or higher than 9.The three strains were capable of util... |
| Keywords: | Gordonia sp polycyclic aromatic hydrocarbons(PAHs) pyrene biodegradation |
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