De novo sequence analysis of cytochrome P450 1–3 genes expressed in ostrich liver with highest expression of CYP2G19 |
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| Authors: | Yusuke K Kawai Kensuke P Watanabe Akihiro Ishii Aiko Ohnuma Hirofumi Sawa Yoshinori Ikenaka Mayumi Ishizuka |
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| Institution: | 1. Laboratory of Toxicology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan;2. Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japan;3. Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo, Japan;1. Faculty of Veterinary Science, The University of Sydney, Sydney, NSW, Australia;2. Discipline of Pharmacology, Sydney Medical School, The University of Sydney, Sydney, NSW, Australia;3. School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW, Australia;1. Toxicology Department, Iuliu Ha?ieganu University of Medicine and Pharmacy, Faculty of Pharmacy, Cluj-Napoca, Romania;2. Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg;3. Health Unit, Flemish Institute for Technological Research (VITO NV), Mol, Belgium;1. Department of Pathology, The University of Hong Kong, Hong Kong;2. State Key Laboratory for Liver Research, The University of Hong Kong, Hong Kong |
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| Abstract: | The cytochrome P450 (CYP) 1–3 families are involved in xenobiotic metabolism, and are expressed primarily in the liver. Ostriches (Struthio camelus) are members of Palaeognathae with the earliest divergence from other bird lineages. An understanding of genes coding for ostrich xenobiotic metabolizing enzyme contributes to knowledge regarding the xenobiotic metabolisms of other Palaeognathae birds. We investigated CYP1–3 genes expressed in female ostrich liver using a next-generation sequencer. We detected 10 CYP genes: CYP1A5, CYP2C23, CYP2C45, CYP2D49, CYP2G19, CYP2W2, CYP2AC1, CYP2AC2, CYP2AF1, and CYP3A37. We compared the gene expression levels of CYP1A5, CYP2C23, CYP2C45, CYP2D49, CYP2G19, CYP2AF1, and CYP3A37 in ostrich liver and determined that CYP2G19 exhibited the highest expression level. The mRNA expression level of CYP2G19 was approximately 2–10 times higher than those of other CYP genes. The other CYP genes displayed similar expression levels. Our results suggest that CYP2G19, which has not been a focus of previous bird studies, has an important role in ostrich xenobiotic metabolism. |
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