Caspase 3, periodically expressed and activated at G2/M transition, is required for nocodazole-induced mitotic checkpoint |
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Authors: | S.-L. Hsu C. -T. R. Yu S. -C. Yin M. -J. Tang A. -C. Tien Y. -M. Wu C. -Y. F. Huang |
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Affiliation: | (1) Department of Education and Research, Taichung Veterans General Hospital, Taichung, 407, Taiwan;(2) Institute of Public Health School of Medicine, National Yang-Ming University, Taipei, 112, Taiwan, R.O.C;(3) Graduate Institute of Clinical Medicine and Department of Physiology, National Cheng Kung University Medical College, Tainan, 701, Taiwan;(4) National Cancer Research Center, National Health Research Institutes, Taipei, 115, Taiwan;(5) Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 114, Taiwan, R.O.C.;(6) Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, 112, Taiwan, R.O.C.;(7) National Cancer Research Center, National Health Research Institutes, 9F. Room 9323, No. 161, Sec. 6, Min-Chuan East Road, Taipei, 114, Taiwan,, R.O.C |
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Abstract: | ![]() Caspases have been known for several years for their involvement in executing apoptosis, where unwanted or damaged cells are eliminated. Surprisingly, after analysis of the relevant data set from the Stanford microarray database, we noticed that the gene expression pattern for caspase 3, but not for caspase 1, 6, 7, 8, 9, or 10, undergoes periodic change in the HeLa cell cycle. In this study, we have demonstrated that caspase 3, but not other caspases, is upregulated and activated just prior to mitosis. Pretreatment of human hepatoma cells with a caspase 3 inhibitor z-DEVD-FMK, prior to the treatment with an antimicrotubule drug nocodazole, abrogates the mitotic arrest, suggesting that caspase 3 (or a caspase 3-like enzyme) might be involved in mitotic-spindle checkpoint. The studies not only characterize caspase 3 as a cell cycle-regulated protein, but also link the protein to nocodazole-dependent mitotic checkpoint, greatly expanding the understanding of caspase 3. These authors contributed equally. |
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Keywords: | caspase 3 mitotic checkpoint nocodazole |
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