Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. E1 |
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Authors: | Bihari Zoltán Szabó Zsolt Szvetnik Attila Balázs Margit Bartos Péter Tolmacsov Péter Zombori Zoltán Kiss István |
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Institution: | Department of Applied Microbiology, Institute for Biotechnology, Bay Zoltán Foundation for Applied Research, Derkovits fasor 2., H-6726 Szeged, Hungary. bihari@bay.u-szeged.hu |
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Abstract: | The newly isolated strain E1, identified as a Dietzia sp., proved to have an excellent ability to degrade n-C12 to n-C38 alkane components of crude oil. The preferred substrate was the very long-chain alkane n-eicosane at an optimal temperature of 37 degrees C and an optimal pH of 8 under aerobic conditions. The growth and substrate uptake kinetics were monitored during the n-alkane fermentation process, and Dietzia sp. E1 cells were found to possess three distinct levels of cell-surface hydrophobicity. Gas chromatographic/mass spectrometric analysis revealed that intracellular substrate mineralization occurred through the conversion of n-alkane to the corresponding n-alkanal. The monoterminal oxidation pathway was presumably initiated by AlkB and CYP153 terminal alkane hydroxylases, both of their partial coding sequences were successfully detected in the genome of strain E1, a novel member of the Dietzia genus. |
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