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Genetic Recombination — Understanding the Mechanism : by H.L.K. Whitehouse Wiley; Chichester,New York, 1982 |
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| Authors: | DB Roberts |
| Institution: | Department of Chemistry, New Mexico State University, Las Cruces, NM 88003, USA |
| Abstract: | Interaction of peanut agglutinin with MeUmbβGalβ(1→3)GalNAc was followed with the stopped-flow technique. The mechanism is a simple bimolecular association with k+ = 7.1 × 103 M?1. s?1 and k? = 0.24 s?1 at 25°C. The very slow dissociation rate of the complex strongly supports earlier conclusions that the combining site of peanut agglutinin is complementary to the Galβ(1→3)GalNac structure. |
| Keywords: | Lectin Peanut Carbohydrate Binding site Difference absorption Stopped flow Me α-Gal methyl α-D-galactopyranoside Me β-Gal methyl β-D-galactopyranoside Me-UmbβGalβ(1→3)GalNAc PNA peanut agglutinin |
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