The secondary attachment site for bacteriophage lambda in the proA/B gene of Escherichia coli期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

The secondary attachment site for bacteriophage lambda in the proA/B gene of Escherichia coli

Authors:	J L Pinkham T Platt L W Enquist R A Weisberg

Affiliation:	Department of Molecular Biophysics and Biochemistry Yale University School of Medicine New Haven, CT 06510, U.S.A.;Laboratory of Molecular Virology National Cancer Institute National Institutes of Health Bethesda, MD 20205, U.S.A.;Laboratory of Molecular Genetics National Institute of Child Health and Human Development National Institutes of Health Bethesda, MD 20205, U.S.A.

Abstract:	We have determined the nucleotide sequence of a secondary λ attachment site in $pro A B$ , a site that accounts for 3% of lysogens isolated from Escherichia coli strains deleted for the primary site. Direct sequence analysis of the transducing bacteriophages carrying the left and right att junctions, as well as the recombinant pro⁺ phage reveals that the $pro A B$ site shares an 11-nucleotide interrupted homology with the core sequence of the primary site. We have compared the $pro A B$ att site with other secondary attachment sites to gain insights into the structural features important for λ integration.

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