In vivo analysis of straight-chain and branched-chain fatty acid biosynthesis in three actinomycetes |
| |
| Authors: | Kimberlee K. Wallace Bitao Zhao Hamish A.I. McArthur Kevin A. Reynolds |
| |
| Affiliation: | Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland at Baltimore, Baltimore, MD 21201, USA; Central Research Division, Pfizer Incorporated, Groton, CT 06340, USA |
| |
| Abstract: | Abstract The starter units for branched-chain and straight-chain fatty acid biosynthesis was investigated in vivo in three actinomycetes using stable isotopes. Branched-chain fatty acids, which constitute the majority of the fatty acid pool, were confirmed to be biosynthesized using the amino acid degradation products methylbutyryl-CoA and isobutyryl-CoA as starter units. Straight-chain fatty acids were shown to be constructed using butyryl-CoA as a starter unit. Isomerization of the valine catabolite isobutyryl-CoA was shown to be only a minor source of this butyryl-CoA. |
| |
| Keywords: | Actinomycete Butyrate metabolism Fatty acid biosynthesis Amino acid catabolism |
|
|
